ENDOGAMY MARRIAGE MITOCHONDRIAL DNA VARIATION OF NORTH CIGINTUNG GARUT ISOLATES

Rina Budi Satiyarti, Rindu Anggita

Abstract


Mitochondrial DNA (mtDNA) has an unique genetic sequences which made its different from nuclear DNA. MtDNA is maternally inherited without recombination, and it has higher mutation rate rather than nuclear DNA. D-loop is an noncoding area in mtDNA which has highest polymorphism among mtDNA. The objective of this research is to study about nucleotide variation in D-Loop area from people who has endogamy marriage. The subject is four generation of maternally inherited. The research steps are sample collection, DNA template preparation, mtDNA amplification, agarose gel electrophoresis, sequencing analysis and homology analysis. The amplification of D-Loop fragment has 0,9 kb in size. Homology analysis showed fifty varians nucleotides. 


Keywords


Mitochondrial DNA (mtDNA), D-loop, nucleotides variation

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DOI: http://dx.doi.org/10.24042/biosf.v9i1.2883

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