Effects of Tapak Dara Leaves Extract on Kidney Histology of Aspartame-Induced Mice

Eva Tyas Utami, Indah Salsabila Kurnia, Mahriani Mahriani

Abstract


This study aimed to determine the effect of Catharanthus roseus leaves extract on kidney histology of mice aspartame-induced. Mice were divided into 4 groups, namely the negative control group (K-) without treatment, the positive control (K+) given aspartame 0.02 mg/day, the dose group 1 (D1) was given aspartame 0.02 mg/day and continued with the C. roseus leaves extract at a dose of 0.42 mg/kg. Group 2 (D2) was given aspartame 0.02 mg/day followed by C. roseus leaf extract at a dose of 0.84 mg/kg BW. IP administered aspartame for 14 days, and C. roseus leaves extract was administered by gavage for 12 days. The data obtained were analyzed by One Way ANOVA and DMRT test with a significance level of 0.05. The results showed that the induction of aspartame at a dose of 0.02 mg/day in mice increased the average number of proximal tubular necrosis cells. Catharanthus roseus leaves extract administration at 0.42 mg/kg BW and 0.84 mg/kg BW for 12 days can reduce the number of proximal tubular necrosis cells in aspartame-induced mice. Thus it can be concluded that the administration of C. roseus leaves extract can reduce the proximal kidney tubules necrosis but has not been able to reach normal conditions yet.

Keywords


Aspartame; Catharanthus roseus; Kidney; Necrosis.

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References


Abechi, S. E., Abbas, N. S., & Gerald, I. U. (2014). Evaluation of caffeine, aspartame and sugar contents in energy drinks. J Chem Pharm Res, 6, 39–43.

Aisyah, S. N. (2018). Histopatologi Hepar Mencit (Mus Musculus) Strain Balb/C Setelah Pemberian Ekstrak Etanol Daun Tapak Dara (Catharanthus Roseus).

Akhlaghi, M., & Bandy, B. (2009). Mechanisms of flavonoid protection against myocardial ischemia–reperfusion injury. Journal of Molecular and Cellular Cardiology, 46(3), 309–317.

Arifin, B., & Ibrahim, S. (2018). Struktur, bioaktivitas dan antioksidan flavonoid. Jurnal Zarah, 6(1), 21–29.

Barrera, G. (2012). Oxidative stress and lipid peroxidation products in cancer progression and therapy. International Scholarly Research Notices, 2012.

Basith, A., Listyaningsih, E., & Handayani, S. (2016). Gambaran Histologis Sel Ginjal Mencit (Mus musculus) yang diinduksi Parasetamol ditambahkan Ekstrak Daun Kemangi (Ocimum sanctum). Syifa’MEDIKA: Jurnal Kedokteran Dan Kesehatan, 7(1), 1–8.

Berawi, K. N., & Agverianti, T. (2017). Efek Aktivitas Fisik pada Proses Pembentukan Radikal Bebas sebagai Faktor Risiko Aterosklerosis. Jurnal Majority, 6(2), 86–91.

BPOM, B. (2014). Peraturan Kepala Badan Pengawas Obat dan Makanan RI N0.12 tentang Persyaratan Mutu Obat Tradisional.

Chinnala, K. M., Elsani M, M., Kotte, S. R., & Sivaramiah, N. (2014). Evaluation of Hepatoprotective Activity of Catharanthus roseus Linn against Paracetamol Induced Hepatotoxicity in Rats. Int.J.Inv. Pharm.Sci, 2(1), 593–599.

Dali, S., Kusuma, A. T., & Anar, A. W. (2013). Analisis kandungan aspartam yang terdapat pada minuman jajanan anak sekolah yang beredar di makassar dengan metode HPLC. Jurnal Ilmiah As-Syifaa, 5(2), 162–168.

Deakandi, W. Y., Risandiansyah, R., & Yahya, A. (2017). Pengaruh Dekokta Eceng gondok (Eichhornia crassipes) terhadap Kadar Malondialdehid (MDA) dan Nekrosis Sel Tubulus Proksimal Ginjal Tikus Wistar Jantan dengan Induksi Oral Kadmium Klorida (CdCl2) Subkronis Dosis Rendah. JIMR-Journal of Islamic Medicine Research, 1(1).

Dewi, S. R., Argo, B. D., & Ulya, N. (2018). Kandungan flavonoid dan aktivitas antioksidan ekstrak Pleurotus ostreatus. Rona Teknik Pertanian, 11(1), 1–10.

Dhalila, H., Zulfitri, Z., & Sofia, S. (2017). Efek Formalin terhadap Jumlah Sel Spermatogenik. Kes Mas: Jurnal Fakultas Kesehatan Masyarakat Universitas Ahmad Daulan, 11(1), 72–77.

Eroschenko, V. P. (2008). Atlas Histology diFiore dengan Korelasi Fungsional edisi. 11th. Diterjemahkan oleh BU Pendit (11th ed.). EGC Penerbit Buku Kedokteran.

Feehley, T., & Nagler, C. R. (2014). The weighty costs of non-caloric sweeteners. Nature, 514(7521), 176–177.

Felton, L. A. (2006). A Review of:“Handbook of Pharmaceutical Excipients, ” RC Rowe, PJ Sheskey, and SC Owen, editors, Pharmaceutical Press, 2005, Hardback, 928, 0853696187. Taylor & Francis.

Fitrianingsih, S. P. (2010). Aktivitas Antiplasmodium Ekstrak Etanol Beberapa Tanaman Obat Terhadap Mencit Yang Diinfeksi Plasmodium berghei. Prosiding SNaPP: Sains, Teknologi, 1(1), 1–13.

Gabr, M., El-Meligy, M., Mohamed, H., & Mohamed, O. (2011). The Effects of Chronic Aspartame Administration on the Kidney of Albino Rat. The Egyptian Journal of Anatomy, 34(2), 85–102.

Hadju, N. A., Tuju, T. D., Ludong, M. M., & Langi, T. M. (2013). Analisis zat pemanis buatan pada minuman jajanan yang dijual di pasar tradisional kota Manado. COCOS, 2(1).

Hamidah, M., & Yulianti, K. (2017). Temuan Post Mortem Akibat Keracunan Metanol. E-Jurnal Medika, 6(7), 1–7.

Irawati, U. B. (2007). Pengaruh aspartam terhadap kadar treatinin serum dan struktur histologis ren mencit (Mus Musculus L.) strain swiss.

Irmawati, F., & Primiani, C. N. (2017). Perbandingan Uji Toksisitas Fitoestrogen Pada Ginjal Tikus (Sprangue Dawley) Yang Diinduksi Daidzein Dan Air Perasan Umbi Bengkuang (Pachyrhizus erosus). Bioeksperimen: Jurnal Penelitian Biologi, 3(2), 52–60.

Kapoor, M., & Rani, J. (2019). Determination Of Vindoline And Rutin Content In Five Different Morphotypes Of Catharanthus Roseus Leaves Using Hplc. Int J Pharm Pharm Sci.

Khrestyawan, L. (2010). Pengaruh Pemberian Ekstrak Biji Kakao (Theobroma cacao) terhadap Kerusakan Sel Ginjal Mencit (Mus musculus) yang Diinduksi Parasetamol. Skripsi. Surakarta: Fakultas Kedokteran, Universitas Sebelas Maret.

Kumar, V., Cotran, R. S., & Robbins, S. L. (2007). Buku ajar patologi. Edisi Ke-7. Jakarta: EGC.

Kurniadi, E., Rousdy, D. W., & Yanti, A. H. (2018). Aktivitas nefroprotektif ekstrak metanol buah lakum (Cayratia trifolia (L.) Domin) terhadap induksi parasetamol. Jurnal Labora Medika, 2(1), 14–21.

Kusuma, W. (2010). Efek Ekstrak Daun Kemangi (Ocimum sanctum L.) terhadap Kerusakan Hepatosit Mencit Akibat Minyak Sawit dengan Pemanasan Berulang.

Madboly, A. G., Metwally, E. S., & Abd El-Fattah, G. A. (2019). Nephrotoxicity of Tow Food Additives (Aspartame and Monosodium Glutamate) in Adult Albino Rats: Biochemical and Histopathological Effects.

Maiese, K., Chong, Z. Z., Hou, J., & Shang, Y. C. (2010). Oxidative stress: Biomarkers and novel therapeutic pathways. Experimental Gerontology, 45(3), 217–234.

Maisaroh, S. (2021). Pengaruh rebusan simplisia daun pulutan (Urena lobata L.) terhadap nekrosis sel tubulus kontortus proksimal ginjal mencit (Mus musculus) galur Balb C. SKRIPSI Mahasiswa UM.

Mayori, R., Marusin, N., & Tjong, D. H. (2013). Pengaruh pemberian Rhodamin B terhadap struktur histologis ginjal mencit putih (Mus musculus L.). Jurnal Biologi Unand, 2(1).

Othman, S. I., & Bin-Jumah, M. (2019). Histopathological effect of aspartame on liver and kidney of mice. International Journal of Pharmacology, 15(3), 336–342.

Parthasarathy, N. J., Kumar, R. S., Manikandan, S., & Devi, R. S. (2006). Methanol-induced oxidative stress in rat lymphoid organs. Journal of Occupational Health, 48(1), 20–27.

Procházková, B., & Bousova, I. (2019). Wilhelmová. 2011. Fitoterapia Antioxidant and Prooxidant Properties of Fl Flavonoids, 82(524), 513–523.

Redha, A. (2010). Flavonoids: Structure, Antioxidative Properties and Their Role in Biological Systems. Belian’s Journal, 2(9).

Rohmani, A., Djamil, S. L., & Indah, A. R. (2015). Efek Toksik Formalin terhadap Gangguan Fungsi Hepar. Jurnal Kedokteran Muhammadiyah, 4.

Situmeang, R. M., & Sudharmono, U. (2019). Effectiveness Rosy Periwinkle (Catharanthus roseus L.) Decoction Toward Urea and Creatinine Serum Levels of Male Wistar Strain Rats with Acute Kidney Failure Model. Abstract Proceedings International Scholars Conference, 7(1), 768–779.

Sumardika, I. W., & Jawi, I. M. (2012). Ekstrak air daun ubi jalar ungu memperbaiki profil lipid dan meningkatkan kadar SOD darah tikus yang diberi makanan tinggi kolesterol. Medicina, 43(2), 67–71.

Susatiningsih, T. (2015). Obesitas dan Stress Oksidatif. JuKe University of Lampung, 5(9), 89–93.

Susianti, S., Suwandi, J. F., & Firtanti, A. (2010). Pembengkakan Sel Tubulus Proksimal Ginajl Mencit (Mus musculus L.) Jantan Galur Balb/C Akibat Pemberian Ekstrak Buah Mahkota dewa (Phaleria macrocarpa (Scheff.) Boerl.). Proceedings of the National Seminar on Science & Technology – III, 449–457.

Sweetman, S. (2009). Martindale The Complete Drug Reference (Thirty-sixth ed). Pharmaceutical Press.

Verrananda, I., Yulita, V., Febriana, L., & Rijai, L. (2019). Identifikasi Metabolit Sekunder dan Aktivitas Antioksidan Ekstrak Bunga Tapak Dara (Catharanthus roseus). Proceedings of the 4th National Pharmaceutical Seminar, 1, 162–167.

Wahyuono, S., Widyarini, S., & Yuswanto, Y. (2017). Aktivitas Antioksidan Buah Kopi Hijau Merapi. JPSCR: Journal of Pharmaceutical Science and Clinical Research, 2(2), 130–136.

Widayati, E. (2021). Oxidasi biologi, radikal bebas, dan antioxidant. Majalah Ilmiah Sultan Agung, 50(128), 26–32.

Wiratmoko, W., & Nurmalasari, Y. (2014). Analisis Jenis Minuman Ringan Yang Mengandung Aspartam Terhadap Kese Hatan Siswa di Sekolah Dasar Negeri 2 Raja Basa Bandar Lampung. Jurnal Ilmu Kedokteran Dan Kesehatan, 1(4).

Wirawan, W. (2018). Test of ethanol extract of ciplukan leaves on the kidney histopathological picture of male white rats induced by streptozotocin. . 15,2:124-133. Pharmacology Journal of Pharmacy, 15(2), 124–133.

Worotikan, R. V., Tuju, E. A., & Kawuwung, F. (2017). Analisis Efektivitas Antidiabetes Ekstrak Etanol Buah Andaliman (Zanthoxylum acanthopodium DC) pada Histopatologi Ginjal Tikus Putih (Rattus novergicus) yang Diinduksi Alloksan. Jurnal Sains, Matematika Dan Edukasi (JSME), 5(1), 29–37.




DOI: http://dx.doi.org/10.24042/biosfer.v12i2.10007

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